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PageRuler Plus Prestained Protein Ladder, 10 to 250kDa
| 品牌： | Fermentas |
|---|---|
| 目录价： | 4754 |
| 优惠价： | |
| 库存货期： | |
| 其他名称： | 26620 预染蛋白Marker 10~250kDa |
| 产品官网链接： |
| 目录号： | 26620 |
|---|---|
| 规格： | 10 X 250 UL |
| 运输温度： | |
| 保存温度： | |
| 说明书： | 阅读 |
| 数量： |
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描述
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.Features of PageRuler Plus Prestained Protein Ladder:
• Size range—nine proteins spanning 10 to 250kDa
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—color-coded bands of similar intesity for easy visualization
• Quality tested—each lot evaluated by SDS-PAGE and Western blotting
• Bright reference bands—orange at 70 and 25kDa, and green at 10kDa
• Membrane-compatible—colored bands transfer to membranes for Western blotting
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.
规格
| Stain Type: | Prestained |
|---|---|
| System: | SDS-PAGE |
| Product Size: | 10 x 250 µL |
内容及储存
Upon receipt store at -20°C. Product is shipped with an ice pack.
Migration patterns of PageRuler Plus Prestained Protein Ladder in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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Migration patterns of PageRuler Plus Prestained Protein Ladder in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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